clustalw implemented in bioedit version 7.2.6 (Bioedit Company)
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Clustalw Implemented In Bioedit Version 7.2.6, supplied by Bioedit Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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1) Product Images from "Novel duplex TaqMan-based quantitative PCR for rapid and accurate diagnosis of Leishmania ( Mundinia ) martiniquensis and Leishmania ( Mundinia ) orientalis , responsible for autochthonous leishmaniasis in Thailand"
Article Title: Novel duplex TaqMan-based quantitative PCR for rapid and accurate diagnosis of Leishmania ( Mundinia ) martiniquensis and Leishmania ( Mundinia ) orientalis , responsible for autochthonous leishmaniasis in Thailand
Journal: Current Research in Parasitology & Vector-borne Diseases
doi: 10.1016/j.crpvbd.2024.100217
Figure Legend Snippet: A Schematic representation of the rRNA transcription unit encoding 18S rRNA, ITS1, 5.8S rRNA, ITS2, and 28S rRNA in Leishmania species. B ClustalW alignment of the 5′-end of the ITS1 region shows two highly conserved sequence blocks used to design forward and reverse primers for amplification of L. martiniquensis , generating an amplicon of 116 bp. The LmarITS1 probe was then designed based on the sequence found exclusively in L. martiniquensis .
Techniques Used: Sequencing, Amplification
Figure Legend Snippet: A Schematic representation of the intergenic region between two Leishmania HSP70-I loci. B ClustalW alignment of the HSP70-I IR revealed the sequence region used to design primers and a probe for amplification of L. orientalis with a product size of 64 bp. Note that the closely related L. chancei could potentially be amplified using primers and a probe designed for L. orientalis in this study.
Techniques Used: Sequencing, Amplification