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Bioedit Company clustalw implemented in bioedit version 7.2.6
A Schematic representation of the rRNA transcription unit encoding 18S rRNA, ITS1, 5.8S rRNA, ITS2, and 28S rRNA in Leishmania species. B <t>ClustalW</t> alignment of the 5′-end of the ITS1 region shows two highly conserved sequence blocks used to design forward and reverse primers for amplification of L. martiniquensis , generating an amplicon of 116 bp. The LmarITS1 probe was then designed based on the sequence found exclusively in L. martiniquensis .
Clustalw Implemented In Bioedit Version 7.2.6, supplied by Bioedit Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Novel duplex TaqMan-based quantitative PCR for rapid and accurate diagnosis of Leishmania ( Mundinia ) martiniquensis and Leishmania ( Mundinia ) orientalis , responsible for autochthonous leishmaniasis in Thailand"

Article Title: Novel duplex TaqMan-based quantitative PCR for rapid and accurate diagnosis of Leishmania ( Mundinia ) martiniquensis and Leishmania ( Mundinia ) orientalis , responsible for autochthonous leishmaniasis in Thailand

Journal: Current Research in Parasitology & Vector-borne Diseases

doi: 10.1016/j.crpvbd.2024.100217

A Schematic representation of the rRNA transcription unit encoding 18S rRNA, ITS1, 5.8S rRNA, ITS2, and 28S rRNA in Leishmania species. B ClustalW alignment of the 5′-end of the ITS1 region shows two highly conserved sequence blocks used to design forward and reverse primers for amplification of L. martiniquensis , generating an amplicon of 116 bp. The LmarITS1 probe was then designed based on the sequence found exclusively in L. martiniquensis .
Figure Legend Snippet: A Schematic representation of the rRNA transcription unit encoding 18S rRNA, ITS1, 5.8S rRNA, ITS2, and 28S rRNA in Leishmania species. B ClustalW alignment of the 5′-end of the ITS1 region shows two highly conserved sequence blocks used to design forward and reverse primers for amplification of L. martiniquensis , generating an amplicon of 116 bp. The LmarITS1 probe was then designed based on the sequence found exclusively in L. martiniquensis .

Techniques Used: Sequencing, Amplification

A Schematic representation of the intergenic region between two Leishmania HSP70-I loci. B ClustalW alignment of the HSP70-I IR revealed the sequence region used to design primers and a probe for amplification of L. orientalis with a product size of 64 bp. Note that the closely related L. chancei could potentially be amplified using primers and a probe designed for L. orientalis in this study.
Figure Legend Snippet: A Schematic representation of the intergenic region between two Leishmania HSP70-I loci. B ClustalW alignment of the HSP70-I IR revealed the sequence region used to design primers and a probe for amplification of L. orientalis with a product size of 64 bp. Note that the closely related L. chancei could potentially be amplified using primers and a probe designed for L. orientalis in this study.

Techniques Used: Sequencing, Amplification



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Bioedit Company clustalw implemented in bioedit version 7.2.6
A Schematic representation of the rRNA transcription unit encoding 18S rRNA, ITS1, 5.8S rRNA, ITS2, and 28S rRNA in Leishmania species. B <t>ClustalW</t> alignment of the 5′-end of the ITS1 region shows two highly conserved sequence blocks used to design forward and reverse primers for amplification of L. martiniquensis , generating an amplicon of 116 bp. The LmarITS1 probe was then designed based on the sequence found exclusively in L. martiniquensis .
Clustalw Implemented In Bioedit Version 7.2.6, supplied by Bioedit Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/clustalw+implemented+in+bioedit+version+7%2E2%2E6/clustalw+in+bioedit/pmc11619792-135-11-14
Average 90 stars, based on 1 article reviews
clustalw implemented in bioedit version 7.2.6 - by Bioz Stars, 2026-09
90/100 stars
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Bioedit Company clustalw implemented in bioedit sequence alignment editor version 7.2.6
A Schematic representation of the rRNA transcription unit encoding 18S rRNA, ITS1, 5.8S rRNA, ITS2, and 28S rRNA in Leishmania species. B <t>ClustalW</t> alignment of the 5′-end of the ITS1 region shows two highly conserved sequence blocks used to design forward and reverse primers for amplification of L. martiniquensis , generating an amplicon of 116 bp. The LmarITS1 probe was then designed based on the sequence found exclusively in L. martiniquensis .
Clustalw Implemented In Bioedit Sequence Alignment Editor Version 7.2.6, supplied by Bioedit Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/clustalw+implemented+in+bioedit+version+7%2E2%2E6/clustalw+in+bioedit/pmc08877104-60-9-13
Average 90 stars, based on 1 article reviews
clustalw implemented in bioedit sequence alignment editor version 7.2.6 - by Bioz Stars, 2026-09
90/100 stars
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A Schematic representation of the rRNA transcription unit encoding 18S rRNA, ITS1, 5.8S rRNA, ITS2, and 28S rRNA in Leishmania species. B ClustalW alignment of the 5′-end of the ITS1 region shows two highly conserved sequence blocks used to design forward and reverse primers for amplification of L. martiniquensis , generating an amplicon of 116 bp. The LmarITS1 probe was then designed based on the sequence found exclusively in L. martiniquensis .

Journal: Current Research in Parasitology & Vector-borne Diseases

Article Title: Novel duplex TaqMan-based quantitative PCR for rapid and accurate diagnosis of Leishmania ( Mundinia ) martiniquensis and Leishmania ( Mundinia ) orientalis , responsible for autochthonous leishmaniasis in Thailand

doi: 10.1016/j.crpvbd.2024.100217

Figure Lengend Snippet: A Schematic representation of the rRNA transcription unit encoding 18S rRNA, ITS1, 5.8S rRNA, ITS2, and 28S rRNA in Leishmania species. B ClustalW alignment of the 5′-end of the ITS1 region shows two highly conserved sequence blocks used to design forward and reverse primers for amplification of L. martiniquensis , generating an amplicon of 116 bp. The LmarITS1 probe was then designed based on the sequence found exclusively in L. martiniquensis .

Article Snippet: Representative sequences of each locus from Leishmania spp. were aligned using ClustalW implemented in BioEdit version 7.2.6 ( ) to reveal the regions of high intraspecific conservation, which allowed us to design custom primers and probes for the duplex qPCR assay, as illustrated in , .

Techniques: Sequencing, Amplification

A Schematic representation of the intergenic region between two Leishmania HSP70-I loci. B ClustalW alignment of the HSP70-I IR revealed the sequence region used to design primers and a probe for amplification of L. orientalis with a product size of 64 bp. Note that the closely related L. chancei could potentially be amplified using primers and a probe designed for L. orientalis in this study.

Journal: Current Research in Parasitology & Vector-borne Diseases

Article Title: Novel duplex TaqMan-based quantitative PCR for rapid and accurate diagnosis of Leishmania ( Mundinia ) martiniquensis and Leishmania ( Mundinia ) orientalis , responsible for autochthonous leishmaniasis in Thailand

doi: 10.1016/j.crpvbd.2024.100217

Figure Lengend Snippet: A Schematic representation of the intergenic region between two Leishmania HSP70-I loci. B ClustalW alignment of the HSP70-I IR revealed the sequence region used to design primers and a probe for amplification of L. orientalis with a product size of 64 bp. Note that the closely related L. chancei could potentially be amplified using primers and a probe designed for L. orientalis in this study.

Article Snippet: Representative sequences of each locus from Leishmania spp. were aligned using ClustalW implemented in BioEdit version 7.2.6 ( ) to reveal the regions of high intraspecific conservation, which allowed us to design custom primers and probes for the duplex qPCR assay, as illustrated in , .

Techniques: Sequencing, Amplification